Description

Technical Parameters

1. What is Procyanidin B2?

Procyanidin is a natural polyphenolic compound, which is one of the most powerful free radical scavengers discovered so far. It has powerful anti-oxidation and anti-non-enzymatic glycation activity, and has a strong cardiovascular protective effect. As a natural plant component, it shows no significant toxicity and is widely used globally. Among them, dimers are the most widely distributed among various proanthocyanidins and have the strongest antioxidant activity. Procyanidin B2 is the main component of GSPE, and the activity of Procyanidin B2 is the strongest among the eight isomers. Numerous studies have demonstrated that procyanidin exhibits significantly stronger antioxidant and free radical-scavenging capabilities than vitamins E and C. It helps prevent and treat various diseases linked to free radical damage, including heart disease and arthritis, and also improves microcirculation.

Abbr.: OPC-B2

Solubility: Slightly soluble in water, easily soluble in organic solvents such as methanol, ethanol, acetone, etc

Molecular formula: C30H26O12

Molecular weight: 578.52

Odor and taste: Characteristic

Structural formula:

Procyanidin_B2 Structural formula

2. Certificate of Analysis(COA)

Product Name	Procyanidin B2	Botanical Source	Malus pumila Mill.
CAS No.	29106-49-8	Plant Part	Fruit
ITEM	SPECIFICATION	RESULT	TEST METHOD
Physical Description
Appearance	Light Brown or Brownish Yellow Powder	Conforms	Visual
Odor	Characteristic	Conforms	Organoleptic
Taste	Characteristic	Conforms	Olfactory
Particle size	95% passes through an 80 mesh sieve	Conforms	CP2015
Chemical Tests
Procyanidin B2	≥8%	8.21%	HPLC
Loss on drying	≤5.0%	3.51%	GB 5009.3 (105 ℃, 3 h)
Ash	≤3.0%	2.11%	GB 5009.4 (600 ℃, 4 h)
Heavy Metals	≤20 ppm	Conforms	GB 5009.74
Lead (Pb)	≤1 ppm	0.010 ppm	GB 5009.12
Arsenic (As)	≤0.5ppm	0.021 ppm	GB 5009.11
Cadmium (Cd)	≤0.1 ppm	0.008 ppm	GB 5009.15
Mercury (Hg)	≤0.5 ppm	0.013 ppm	GB 5009.17
Microbiology Control
Aerobic bacterial count	≤1,000 CFU/g	10 CFU/g	GB 4789.2
Total Yeast & Mold	≤100 CFU/g	<10 CFU/g	GB 4789.15
Escherichia coli	Negative	Conforms	GB 4789.38
Salmonella	Negative	Conforms	GB 4789.4
Conclusion	Conforms to specification
Storage:	Store in a cool, dry, well-ventilated area; keep in tightly sealed containers, preferably full.
Shelf Life	24 months when properly stored.
Status:	Non-irradiation; Non-GMO; No Carrier; Halal & Kosher certified.

3. What is the Process Flow Diagram?

In the industrial production of high-purity and high-activity procyanidin B2, the progressiveness, environmental protection, and economy of its technological process are crucial.

3.1 Raw material pretreatment
After cleaning and removing nuclei, the raw materials are subjected to freeze-drying (Lyophilization) or low-temperature drying, and then crushed to a specific mesh size (such as 40-60 mesh) to increase the contact area with the extraction solvent and improve the extraction efficiency._[1]

3.2 Core Extraction Technology: Subcritical Water Extraction (SWE)
Subcritical water extraction (SWE) has demonstrated significant advantages in the research of procyanidin B2 extraction.

3.3 Traditional and Auxiliary Extraction Methods
Despite the obvious advantages of SWE technology, traditional solvent extraction methods (such as using ethanol-water mixtures) are still used in some productions due to their low equipment requirements and mature technology. Additionally, techniques including ultrasound-assisted extraction (UAE) improve mass transfer and reduce extraction time._[3]

3.4 Purification and refining process
The crude extract, containing impurities such as polyphenols, sugars, and pigments, undergoes purification, including solid-liquid separation, concentration, macroporous adsorption resin process, and drying, to obtain high-purity procyanidin B2._[3]

4. Is procyanidin B2 Effective? How Does It Work?

Free radicals and the resulting lipid peroxidation underlie and contribute to a wide range of diseases, including heart disease, arteriosclerosis, phlebitis, arthritis, allergies, Alzheimer's disease, coronary heart disease, and cancer.

Efficacy and Mechanism of Action

Promote hair growth

Research has found that it can selectively promote the proliferation of hair follicle epithelial cells while inhibiting the activity of PKC isoenzymes associated with the resting phase of hair growth._[4] In addition, it can prolong the growth period of hair and reduce hair loss.

Strong antioxidant properties

The multiple phenolic hydroxyl groups of procyanidin B2 are the source of its strong antioxidant activity. It can directly eliminate various ROS and RNS free radicals, block chain reactions caused by oxidative stress, and protect cell membranes, proteins, and DNA from damage.

Strong anti-inflammatory effect

Research has shown that procyanidin B2 can exert anti-inflammatory effects by regulating key inflammatory signaling pathways. For instance, studies indicate that it inhibits the NLRP3 inflammasome pathway, thereby reducing inflammation.

Antitumor potential

Research has confirmed that procyanidin inhibits various tumor cell types and demonstrates substantial anti-tumor efficacy. It can induce programmed death of many tumor cells by activating the apoptosis signal pathway in cells, thus inhibiting tumor growth._[5]

Yin Huiyong, a researcher at the Key Laboratory of Nutrition Metabolism and Food Safety, Shanghai Institute of Nutrition and Health, Chinese Academy of Sciences, published an online publication titled Polyphenolic Proanthocyanidin-B2 Suppresses Proliferation of Liver Cancer Cells on Redox Biology and Hepatocellular Carcinogenesis through Direct Binding and Inhibiting AKT Activity research paper. Research has revealed that Procyanidin B2 (OPC-B2)-an active compound derived from floral polyphenols-acts as a novel allosteric inhibitor of AKT. It directly binds to AKT, inhibiting its phosphorylation and thereby exerting anti-tumor effects. Key residues Lys297 and Arg86 on the AKT protein are critical for this interaction with OPC-B2. These findings propose a potential treatment strategy for hepatocellular carcinoma (HCC).

NLRP3

5. What Are the Applications of Procyanidin B2?

As a natural antioxidant, Procyanidin B2 is increasingly used in pharmaceuticals, health products, and cosmetics due to its potent free radical-scavenging capacity and cardiovascular benefits. It is also recognized as one of the top ten medicinal botanical compounds.

	Product form	Application
Cosmeceuticals & Personal Care	Hair growth essence, anti-stripping shampoos, scalp care ampoule, anti-aging face cream, eye cream, repair essence, etc.	Utilizing its ability to promote hair growth and strong antioxidant capacity.
Functional Foods & Dietary Supplements	Capsules, tablets, oral liquids, functional beverages, antioxidant gummies, etc.	As an oral supplement, it aims to provide systemic antioxidant support, protect cardiovascular health, enhance immunity, and internally improve skin and hair health.

Procyanidin B2

In Germany and Romania, Procyanidin B2 preparations are used to treat microcirculation diseases.
In France, a preparation for treating periodontal disease with Procyanidin oligomers or monomers as active substances has been invented and patented, and it is also made of grape seed proanthocyanidins.
In Germany, proanthocyanidin preparations for the treatment of alcoholism were granted a patent.

In addition, the anti-wrinkle, whitening, moisturizing, and anti-radiation effects of Procyanidin B2 Apple extract have opened up broad prospects for them in the field of cosmetics. France has developed liposome microcapsule night cream, hair cream, and a mouthwash made of Procyanidin oligomers.

6. Certificates

certificates

Security statement：

Our company guarantees that the goods sent are safe, non-flammable, and non-explosive, and consistent with the declaration. Under normal transportation conditions, all losses caused by the products sent by our company shall be borne by our company.

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Qualification

7. Why Choose APPCHEM?

Our production and equipment

years

APPCHEM (Nasdaq: BON), founded in 2006, is a Nasdaq-listed company that specializes in manufacturing botanical extracts.

certificates

APPCHEM maintains a comprehensive set of certifications, including a China SC License, ISO 9001, ISO 22000, FDA registration, as well as Halal and Kosher certifications.

awards

APPCHEM has received extensive recognition for our innovative strengths, earning numerous awards that demonstrate our creative excellence.

Reference:

[1]W. Wahyudiono, S. Maeda, et al. "EXTRACTION OF PROCYANIDIN B2 FROM APPLE PEEL USING SUBCRITICAL WATER." ASEAN Engineering Journal. [2022-06-01]

[2]Shangjian Li, Jiajia Chen, et al. "Application of Procyanidins from Aronia melanocarpa (Michx.) Elliott in Fresh-Cut Apple Preservation." Horticulturae. [2024-05-25]

[3]Huawei Zhang, Chen Ying. "Preparation of procyanidin B2 from apple pomace and its inhibitory effect on the expression of cyclooxygenase-2 in lipopolysaccharide-treated RAW264.7 macrophages." Bangladesh Journal of Pharmacology. [2011-12-10]

[4]Cosmetic Trends in Dermatology: A Systematic Review of Topical Apple Derivatives in Treating Dermatologic Conditions. SHANGYI FU et al. [2023-10-02]

[5]T. Miura, M. Chiba, et al. "Apple procyanidins induce tumor cell apoptosis through mitochondrial pathway activation of caspase-3." Carcinogenesis. [2007-09-07]

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