Broccoli Extract Powder Glucoraphanin HPLC13% 4478-93-7

Glucoraphanin is a natural glucosinolate compound and one of the most abundant glucosinolates found in cruciferous vegetables. It is primarily present in broccoli seeds, sprouts and mature heads, with broccoli seeds containing 60–100 times more than mature heads, making them the finest source material for glucoraphanin extraction.

Many 'broccoli extract' products on the market are labelled directly as the unstable sulforaphane, or lack precise quantification of this key precursor, resulting in significant variations in activity between batches and poor shelf-life stability. This poses significant challenges for B2B clients in terms of formulation consistency, efficacy validation and international compliance.

To address this, we are launching this 13% Glucoraphanin powder (CAS: 4478-93-7), rigorously quantified via HPLC. It is not merely a raw material, but a standardised Nrf2 precursor solution designed for procurement managers, product development engineers and quality decision-makers in the plant extract industry. This product strictly adheres to leading international pharmacopoeias and the ISO quality management system. Its core value lies in: stable precursor morphology, precise HPLC quantification, activation of gut microbiota, and full traceability. Through this comprehensive product dossier, we are committed to presenting you with a complete chain of quality evidence, from broccoli seeds to the final certificate of analysis, serving as a reliable link in your supply chain.

Physical and Chemical Properties

Safety and Compliance Criteria

This product's safety standards comprehensively reference regulatory requirements from major markets, including China, the European Union, and the United States. Both internal and third-party laboratories have validated all data.

As a stable precursor to sulforaphane, the glucoraphanin content directly influences the final dose of the active ingredient that can be converted. A professional Technical Data Sheet (TDS) not only defines the HPLC content but also explains the rationale behind the different specifications.

Standard specification: HPLC 13% glucoraphanin. This is the mainstream industrial specification for broccoli seed water extracts, with a standard requirement of 13–20 g of glucoraphanin per 100 g. The 13% specification strikes a good balance between raw material cost, conversion efficiency and formulation compatibility.

Key Notes: The choice of product specifications is primarily a matter of striking a balance between cost, conversion efficiency and formulation requirements. Whichever specification you choose, you will benefit from our consistent and reliable assurance regarding raw material sourcing, safety testing and quality control systems.

We undertake to provide a COA compliant with international standards with every batch of product. The development and validation of the testing methods for this product have been carried out in accordance with authoritative standards such as the United States Pharmacopoeia (USP), the European Pharmacopoeia (EP), and the Chinese Pharmacopoeia (CP) to ensure global compliance of the data. Furthermore, every test result on the COA is traceable to the original records and is available for audit and verification by customers or third parties.

Important Note: The COA information provided on this page is for illustrative purposes only and is intended to demonstrate the scope and capabilities of our quality control. For the specific COA document of the actual product (including the exact batch number, HPLC test data and chromatograms), please refer to the final version issued by our sales representative.

We employ a proven industrial-scale extraction process to efficiently extract and purify high-concentration glucoraphanin from premium broccoli seeds. This process has been validated for large-scale production and complies with the requirements for new food ingredients. The diagram below illustrates the key process flow from raw material to finished product.

Raw Material Advantages: Broccoli seeds contain the highest levels of sulforaphane, up to 60–100 times higher than mature broccoli florets. We select broccoli seeds from standardised cultivation in premium growing regions to ensure consistent raw material quality.

Further Information: The flowchart above illustrates the main stages of the process. For more detailed production information regarding specific process parameters, production capacity and customised manufacturing, don't hesitate to get in touch with our sales representatives. We will provide further technical consultation tailored to your specific requirements.

The quality management system we have established is a closed-loop ecosystem covering the entire lifecycle from cultivation and production through to testing and release. At its core lies the principle of 'prevention first, full process control, and data traceability'.

Source Control (Upstream)

• Germplasm Resources: Broccoli breeding has been included in the key R&D projects of the National Major Vegetable Industry Technology System. We collaborate with standardised cultivation bases to ensure the purity of raw material germplasm and the controllability of pesticide residues.
• Raw Material Grading: As broccoli seeds contain the highest levels of glucosinolates, we select only high-quality seeds as raw materials to ensure a solid foundation for glucosinolate content.
• Dual Verification of Incoming Raw Materials: Each batch of raw materials must be accompanied by a certificate of origin. Upon arrival at the facility, the materials first undergo rapid QC testing (initial screening for moisture and glucosinolates). If they pass, they are transferred to the holding warehouse for further comprehensive screening for pesticide residues and heavy metals, ensuring that only high-quality raw materials are used in production.

Production Process Control (Midstream)

• HACCP-based Critical Control Point Management: Multiple Critical Control Points (CCPs) are established along the entire production line. For example, online monitoring is conducted during the extraction (CCP1), concentration (CCP2) and drying (CCP3) processes to ensure that operations remain within the pre-validated 'design space'.
• Clear Batch Management: Strict adherence to clean-out procedures, material identification and electronic batch record systems is enforced to eliminate any potential mix-ups or cross-contamination.

Laboratory Quality Assurance (Downstream)

• Release Testing and Stability Studies: Every batch of finished product must pass comprehensive testing covering all physicochemical and safety parameters. We possess advanced analytical equipment, including HPLC, GC-MS and ICP-MS, to analyse multiple quality parameters such as sulforaphane content, erucic acid residues, moisture, heavy metals and microbiological counts.
• Stability Studies: Long-term and accelerated stability tests are conducted in accordance with the company's internal control guidelines, and the shelf life is determined scientifically. Sulforaphane is a stable solid substance and exhibits excellent shelf-life stability.

Our commitment to transparency in quality and our R&D capabilities are underpinned by the following authoritative certifications, patented technologies and supporting documentation.

Regulations and Certifications:

• New Food Ingredient in China: Water extract of broccoli seeds was approved in 2017 (Announcement No. 7 of the National Health and Family Planning Commission), with a recommended daily intake of ≤1.8 g.

• US FDA GRAS Status: Sulphoraphane has been granted GRAS (Generally Recognised as Safe) status by the US FDA and approved for use as a food additive. System Certification Certificates: FSSC 22000, ISO 9001:2015, ISO 14001, Kosher & Halal certification, FDA-registered facility.
• Research Background: The Johns Hopkins University School of Medicine has collaborated with Brassica Protection Products (BPP) to research and develop sulforaphane and sulforaphane glucoside from broccoli seeds. Hundreds of clinical trials have demonstrated their health benefits in areas such as cancer prevention, detoxification and long-term antioxidant effects. The raw materials for this product are traceable to this high-quality supply chain.

Key Supporting Documents:

• Full Quality Control Report (batch-specific, including HPLC content data, erucic acid residues, heavy metals, microbiological analysis, etc.).
• Material Safety Data Sheet (MSDS).
• Technical Data Sheet (TDS).
• Factory Audit Report Summary (on-site audits can be arranged).
• Allergen declaration.
• Non-GMO declaration.

Access to original documents and in-depth review: We fully recognise the importance of reviewing original documentation during the supplier selection process. Should you require original or certified copies of the above certificates, New Food Ingredient Notification documents, or specific batch support documents for formal supplier evaluation or reference purposes, please contact your dedicated account manager. We will fully cooperate to provide the necessary materials to support your decision-making process.

Based on a well-defined mechanism of action and extensive clinical research data, sulforaphane is an ideal ingredient for the development of multi-target functional products.

Core Functional Applications:

1. Activation of the Nrf2 pathway and cellular protection
2. Intestinal barrier protection
3. Anti-inflammatory and antioxidant effects
4. Obesity and metabolic health
5. Relief of colitis
6. Anti-cancer and antioxidant effects

Recommended Formulation Combinations:

• Gut Health Protocol: Sulforaphane + Bifidobacterium longum/Lactobacillus.
• Sports Nutrition Formula: Sulforaphane + BCAAs/Glutamine + Curcumin.
• Metabolic Health Formula: Sulforaphane + Chromium/Green Tea Extract/Garcinia Cambogia.
• Immune Support Programme: Sulphoraphane + Ganoderma lucidum polysaccharides/yeast β-glucan.

Customised Solutions: We have a dedicated R&D team that can provide bespoke technical support-ranging from formulation recommendations and stability studies to sample production-tailored to your specific product concepts (e.g. gut health synbiotics, Nrf2-activating dietary supplements, metabolic support functional foods).

Contact Us: Should you require a Certificate of Analysis (COA), Technical Data Sheet (TDS), HPLC test data, samples, or wish to discuss customisation requirements for a specific batch, please do not hesitate to contact our technical sales team. We provide free samples for testing; please contact us to arrange delivery before placing an order.

Reference

[1] Dinkova- Kostova A T, Fahey JW, Kostoy R V, et al. KEAPl and done Targeting the NRF2 pathway with sulforaphanel]l. Trends in FoodScience & Technology, 2017, 69(1):257-267.

[2] Momtazmanesh S, Amirimoghaddam-Yazdi Z, Moghaddam HS, et al. Sulforaphane as an adjunctive treatment for irritability in Autism Spectrum Disorder: a randomised, double-blind, placebo-controlled clinical trial: Sulforaphane in treatment of ASD[]l. Psychiatry and Clinical Neurosciences, 2020, 74(7):1-33.

[3]FaheyJ W, Stephenson K K, Wade K L, et al. Urease from Helicobacter pylori is inactivated by sulforaphane and other isothiocyanates. Biochemical & Biophysical Research Communications, 2013, 435(1):1-7.

[4]Sulforaphane-enriched extracts from glucoraphanin-rich broccoli exert antimicrobial activity against gut pathogens in vitro, and innovative cooking methods increase in vivo intestinal delivery of sulforaphane. European Journal of Nutrition, 2020, 60(3):1263- 1276.

[5]Singh K, Connors S L, Macklin E A, et al. Sulforaphane treatment of autism spectrum disorder (ASD). Dl. Proceedings of the National Academy of Sciences of the United States of America, 2014, 111(43):15550.

[6] Harvey CJ, Thimmulappa R K, Sethi S, et al. Targeting Nr2 Signalling Improves Bacterial Clearance by Alveolar Macrophages in Patients with COPD and in a Mouse Model[J]. Science Translational Medicine, 2011, 3(78):78-109.